Stefanie Alexandra Prenn grew up in Vienna and Linz. She received lessons from Georg Baich at the Vienna Conservatory. Then she studied with Tobias Kühne at the University of Music and Performing Arts Vienna. From 2003 to 2007 studies followed at the Conservatorium van Amsterdam with Maarten Mostert and Dmitri Ferschtmann. In 2007 she gave her public diploma concert and graduated with the baccalaureate. Afterwards she continued to study at the University of Music and Performing Arts in Vienna with Reinhard Latzko.
Prenn completed master classes with Sándor Végh, Siegfried Palm, Natalia Gutman, André Emilianoff, Valter Despalj, Ivan Moneghetti, Heidi Litschauer, Paul Gulda among others. She has won numerous awards at national and international cello competitions, such as the Mravinskij competition in St. Petersburg, Russia and the international radio competition Concertino Praga. She gave concerts in venues such as the Wiener Konzerthaus, Konzerthaus Berlin, the Philharmonie St. Petersburg and the Rudolfinum Prague. In 2007 she went on concert tours with the Gustav Mahler Youth Orchestra to the Czech Republic, Japan, Finland and the USA. In collaboration with the composer Georg Nussbaumer, Prenn performed several world premieres (including "parsivalsurvivaltrail", "desert: perforation", "Quanger's quartet cross-section", "butchering: scenes") as a soloist and with the ensemble "Symphoid". She was a scholarship holder of the Carl Michael Ziehrer Foundation and the Emanuel and Sofie Fohn Scholarship Foundation.